Fig 1: Astragaloside IV inhibits NKCC1 expression in hypothalamus during CIR. (A) Statistical chart of NKCC1 level in hypothalamus. Upper panel represents three pairs of western blot samples (n=6 per group, Student's t-test, **P<0.01). (B) Statistical chart of AVP level in pituitary (n=6 per group, Student's t-test, **P<0.01). Saline, the group of rats with CIR and saline pretreatment; Ast, the group of rats with CIR and Astragaloside IV pretreatment. Triangles indicate the individual data obtained from each group. NKCC1, Na+-K+−2Cl− cotransporter isoform 1; CIR, cerebral ischemia reperfusion; AVP, arginine vasopressin.
Fig 2: Upregulation of NKCC1 in hypothalamus-mediated hypertension during CIR. (A) Statistical chart of AVP level in pituitary (n=6 per group, Student's t-test, ***P<0.001). (B) Statistical chart of NKCC1 level in hypothalamus. Upper panel represents three pairs of western blot samples (n=6 per group, Student's t-test, ***P<0.001). (C) Statistical chart of MAP with bumetanide pretreatment (n=10, one-way repeated-measures ANOVA followed by the Holm-Sidak method, ***P<0.001, compared with the baseline before modeling). Sham, the group of rats with sham operation; CIR, the group of rats with CIR. Triangles indicate the individual data obtained from each group. NKCC1, Na+-K+−2Cl− cotransporter isoform 1; CIR, cerebral ischemia reperfusion; AVP, arginine vasopressin; MAP, mean arterial pressure.
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